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Single stage batch adsorber was designed using BBD quadratic model for desired purification.
A single stage batch adsorber was designed using Sips isotherm constants to estimate the amount of carbon required for desired purification.
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A single solvent is not usually effective for the removal of impurities to obtain the desired level of purification (Mishra and Sharma 1999).
It is likely that the stringency of our protocol accounts for the lower coverage and higher specificity of the set of hits retrieved; Modifications of the purification conditions could allow for the desired specificity.
BEMA (β-elimination/Michael addition), takes advantage of the ease of β-elimination of phosphorylated Ser and Thr residues at basic pH and the ability to subject the resulting dehydroalanine/methyl-dehydroalanine products to Michael addition with a desired tag for affinity purification [34 36].
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