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The cells were incubated with compounds for desired length of time.
Measure for desired length.
Repeat Rows 2 and 3 for pattern for desired length.
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We used two logistic regression models: one for migrants' indecision and the other for migrants' desired length of stay.
My visa has already been approved and I was told at the Embassy that to gain admission for my desired length of trip I will need to show a whole lot of proof at Customs of accommodation I have booked, what I plan to do, exactly where I plan to go and when and that I have the funds to finance it.
After transfection the medium was replaced with ligand containing media and treated for the desired length of time.
To detect regions of protein that are under positive selection where the whole protein may not be, one may use a sliding window program, which analyses nucleotide substitution and dn/ds rate ratios for any desired length.
Cells on glass coverslips were treated with 1 µM 4OHT for the desired length of time (0 180 min), fixed in 3% paraformaldehyde/2% sucrose, permeabilized in Triton X-100 solution (0.5% Triton X-100, 20 mM HEPES [pH 7.4], 50 mM NaCl, 3 mM MgCl2, 300 mM sucrose), stained with anti-Myc (9E10) followed by goat anti-mouse IgG rhodamine-conjugated secondary antibody and imaged on a Zeiss microscope.
Validating such a Vout(L) dependence, one legitimates analyzing fibers of shorter length, and using results to compute those for any desired length through linear correlation.
RA FLS cell cultures were starved in serum-free DMEM overnight (total synovial membrane cell cultures were used directly in complete medium) and subsequently subjected to a hypoxic gas mixture (1% oxygen, 5% CO2, 94% N2) for the desired length of time.
Measure for your desired length and width.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com