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I am going to buy myself a hammer and go back and get some chunks to be examined for definitive identification.
Alternatively, as these two species often occupy disparate niches under normal circumstances, BurkDiff can be used as a single-probe assay for definitive identification of B. pseudomallei or B. mallei.
All 7 meningococcal housekeeping genes were readily amplified during MLST, suggesting that M16160 is indeed Nm ST-74566, cc60), and again showing sodC to be a useful tool for definitive identification of carriage isolates.
Using several of these assays in combination for definitive identification could be important, as the Burkholderiaceae are highly recombining organisms [15], [19], [30], and as more and more strains are uncovered, the robustness and sensitivity of these assays will be challenged.
We did not have access to authentic standards for definitive identification of these compounds.
Isolates meeting the preliminary phenotypic characteristics for GPAC should be referred for definitive identification using partial 16S rDNA sequencing.
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We used API ID 32 Staph system (a commercial test system strip consists of 32 cupules, 26 of which contain dehydrated biochemical media for colorimetric tests to identify staphylococci) (Bio-Merieux) for the definitive identification of this bacterium.
These primers were used to develop a rapid PCR assay for the definitive identification of B. mallei and differentiation from all other bacteria.
Flowers were needed for a definitive identification of the species.
chelonae underwent hsp65 and rpoB gene fragment sequencing for more definitive identification.
Full-length sequencing of the 16S rRNA gene was subsequently performed for more definitive identification.
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