Gel-purified PCR products were sent for deep sequencing using the Roche 454 genome sequencer FLX.
The exogenously integrated HBB DNA fragment and 10 potential off-target sites were PCR amplified for deep sequencing.
These results support the use of Illumina as another MPS platform of choice for deep sequencing of viral minority species.
The purified DNA was subjected for either library preparation for deep sequencing (ChIP-seq) or PCR and quantitative PCR (ChIP-PCR and ChIP-qPCR).
Libraries for deep sequencing were prepared from amplified cDNA libraries using previously published protocols [50].
On the one hand, we have added support for deep sequencing (DS) data.
In this study, the polyadenylated transcripts from male and female S. japonicum were isolated for deep sequencing and the sequences were systematically analysed.
A total of 84 RT-PCR clones derived from the p33 ORF of isolate FS2-2 were chosen randomly for deep sequencing analysis.
In particular, the KLS samples were derived from a similar demographic as the H1N1 samples (children and young adults) and were processed identically for deep sequencing.
Small RNA fraction was extracted from the gel slice corresponding to the delimiting markers and used for generating libraries for deep sequencing.
Hence, the inoculated leaves of BaMV-infected and BaMV- and satBaMV-co-infected A. thaliana were harvested at 7 dpi for deep sequencing.
