Sentence examples for for deep preparation from inspiring English sources
If not exactly a charismatic public speaker, he is a master of small meetings and a fearless trader of facts who has earned praise for deep preparation even from the industries regulated by his department.
The purified DNA was subjected for either library preparation for deep sequencing (ChIP-seq) or PCR and quantitative PCR (ChIP-PCR and ChIP-qPCR).
To control for biases in sequencing and mapping, we also mapped nine million published reads from purified genomic DNA ("genomic") that had also been sheared in preparation for deep sequencing [11].
Coverage of the influenza genome may be the best proxy measure for influenza activity and the associated host response in deep sequencing analysis, as it is relatively independent of PCR duplication artifacts that can be introduced during sample preparation for deep sequencing [46].
Sample preparation for deep sequencing using MiSeq followed as previously described [ 9], with an additional centrifugation step at 6,200 g for 10 minutes at 4°C, and DNaseI (PreAnalytiX, QIAGEN, Franklin Lakes, USA) treatment at 37°C for 15 minutes.
All steps including cell growth, RNA isolation and library preparation for deep sequencing were performed in triplicate for the chemical stress samples (except those with threonine, itaconic acid and decanoic acid that were performed once) and in duplicate for the fermentation samples.
Total RNA was extracted using TRIzol reagent (Invitrogen), and 10 μg of each sample were prepared for deep sequencing following Illumina's small RNA sample preparation protocol v1.
Ten micrograms of each sample (roots, flower buds, seedlings, and leaves) were prepared for Deep Sequencing following Illumina's Small RNA alternative sample preparation protocol v1.5.
Instead they drilled a couple of top holes in preparation for deeper drilling next summer.
Indeed, there are reports that indicate that artifactual chimeras could be generated in the RNA sample preparation for deep-sequencing [ 27, 28].
