Sentence examples for for cuticle preparations from inspiring English sources

Homozygotes were then collected for cuticle preparations.

For cuticle preparations, embryos were collected for 24 h at 18°C from w; BicD2/BicDr5 cn; +; att-102F{BicDmutant}/+ mothers, with BicDmutant as indicated in Figure 5.

Unhatched larvae were peeled and transferred to a slide for cuticle preparations in Lacto Hoyer's media.

For cuticle preparations, 24-hr-old embryos were dechorionated in bleach, devitillinized in 1 1 MeOH heptane, and mounted in lactic acid.

For quantification of hatch rates, 300 unhatched embryos were tested per experiment and percentages from two experiments were averaged for the final hatch rate (unfertilized eggs were identified after cuticle preparations and were excluded from the analysis).

Cuticle preparations on embryos that were allowed to develop for more than 24 hours were done by transferring dechorionated embryos into Lacto∶Hoyers (1∶1) on a microscope slide and incubating the slide overnight at 60°C.

Cuticle preparations were as in [67].

Cuticle preparations of embryos were made essentially as described [53].

Neither Rab5DN nor Rab5CA led to disruption of ectodermal epithelial integrity, as assessed by cuticle preparations (Fig. 3B,N,O).

The cuticle preparations were visualized by DIC optics using a 10x 0.3 NA objective on a Leica DM IRE2 inverted microscope and a cooled ORCA ERG 1394 CCD camera (12-bit, 1344×1024 resolution, Hamamatsu Photonics, K.K .. We thank Joao Marques for qPCR confirming absence of increased viral load in the mutant embryos exposed to the T-step relative to controls.

In pilot experiments, flattened adult cuticle preparations were made by macerating the flies in hot 10% KOH solution and placing in a mounting medium (Permount™, Fisher Scientific Research, Pittsburgh PA).