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(C is stand for crosslinked samples).
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The beads were then washed, eluted, loaded onto a 6% Tris-glycine gel for separation, and probed on a PVDF membrane for CEACAM5 as described previously for the anti-CTB IP of crosslinked samples.
Prototype grafts had sufficient burst pressure (>550 mmHg), and compliances of 12.1 ± 0.8 and 6.2 ± 0.3%/100 mmHg for uncrosslinked and crosslinked samples, respectively.
Hereafter, the crosslinked samples are denote by the crosslinking time appended to BR (e.g., BR15 describes a BR film crosslinked for 15 min).
Highly crosslinked samples (Fig. 4) can survive for months in water, and indefinitely in air.
The crosslinked samples exhibit excellent thermal stability and absorb less than 2.7 wt% water after exposure to an aqueous environment for extended periods.
The degree of crosslinking can be estimated from the residual amount of free amine groups of (non-) crosslinked samples.
Next, the purified crosslinked samples were double digested with trypsin and Glu-C protease, fractionated by cation exchange chromatography and analysed by nano-scale reversed phase liquid chromatography coupled to a tandem mass spectrometer for detection and identification of crosslinked peptides.
Having identified candidate fractions after crosslink reversal and SDS-PAGE analysis, we proceeded to dialyze the original crosslinked samples in those fractions against SWR1 buffer containing no glycerol.
The extreme solubility of Gel restricted platelet binding analysis to 10% and 100% EDC crosslinked samples.
This range of [GA] was also the transition zone for changes in the shape of T1ρ dispersion seen in figures 2 and 3. T2/T1 ratios for crosslinked BSA samples dialyzed in excess H2O to remove the possible effects of unreacted GA showed a similar dependence on [GA] as undialyzed samples although the transition zone was narrower.
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