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As an additional reference the variety Barke was included which had been used for creating expression analysis data [ 8].
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Scythe + Sew, in turn, has a set of built in operators for creating expressions for viewing and manipulating data.
A system for direct cloning of gDNA fragments from BACs into a plant transformation vector and creating expression constructs for reporter gene fusions by recombineering is outlined in Figure 3.
... Now, as I read different authors and read different plays, I think I'm more attuned to the voice of the author and the way he creates expression for the characters.
To create expression plasmids for ECFP- and EYFP- tagged PHD2, the PHD2 coding sequence was transferred from pEGFP-N1-PHD2 [29] to pECFP-N1 and pEYFP-N1 (Clontech) by restriction digestion and ligation into the recipient vector.
Using the list of non-synonymous single-nucleotide polymorphisms (SNPs) provided by the AKR website (www.med.upenn.edu/akr), in turn obtained from the dbSNP database[7] at the National Center for Biotechnology Information (NCBI), they created expression constructs for the wild-type (normal) and eleven variants.
Quantifying this we have created expression profiles for the individual libraries, based on the EST coverage along the gene.
Extracted data were imported into Rosetta Resolver 5.1 to create expression profiles for each individual endometrial cell line experiment.
Extracted data were imported into Rosetta Resolver 5.1 (Ceiba, Boston, MA, USA) to create expression profiles for each individual cell line experiment.
To create expression vectors for shRNAi against Bim, HPLC-purified synthetic oligo DNA (see Supplementary Table S7b for sequences) were annealed and ligated into the Aar1 site of the retroviral shRNAi vector L087 (GenBank Acc: EU424173).
The resulting destination vectors GW-pECYFP and GW-pEGFP were used in a Gateway LR reaction along with suitable entry vectors to create expression vectors for CFP-YFP-tagged or GFP-tagged proteins, respectively.
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