Sentence examples for for correct insertion from inspiring English sources

The goals are to insert a DNA fragment of interest into a receiving vector plasmid, transform the plasmid into E. coli, recover the plasmid DNA, and check for correct insertion events.

Following PCR screening for correct insertion and orientation, the kanamycin resistance cassette was introduced into strain SL1344 by bacteriophage P22 transduction using standard protocols.

Shh plasmid was successfully constructed using commercially available pCMV Script Vector using mRNA isolated from 14-day rat embryo and the plasmid construct was sequenced for correct insertion of Shh transgene (Figure S1A & Figure S1B).

Primers used to check for correct insertion: forward 5′-AATAGTAATGAACAAGGTTGTG-3′, reverse 5′-TTGCATTCTATTGTACAACG-3′.

The resulting recombinant pPIC9K– GuHMGR plasmid was transferred into the disarmed E. coli DH5 α and sequenced for correct insertion.

Lipidation of VirB7 is needed for correct insertion of the core complex into the outer membrane and is essential for secretion (Fronzes et al., 2009a).

In the cell lines, however, loss of the docking-site marker and appearance of a marker from the targeting plasmid is not sufficient to give useful enrichment for correct insertions, and it is necessary to include a counterselection, such as GCV, against cells with illegitimate insertions.

The pcDNA3.1 vector containing bursicon α or β cDNA was further sequenced for confirmation of correct insertion.

Bursicon β: forward primer 5'-CTCGAGATGCATGTCCAGGAACTGCT-3'; reverse primer 5'-GGATCCACGTGTGAAATCGCCACATT-3'), cloned into the PGEM-T-Easy vector (Promega), and sequenced again for confirmation of correct insertion.

The most abundant replication products for both adducts were derived for the correct insertion of dCTP, followed by error-free extension.

To allow for a correct insertion of all SELP encoding genes, the original pET-25b expression vector was mutated by site-directed mutagenesis according to the method previously described by Ansaldi, M. et al (Ansaldi et al. 1996).