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This enzyme possesses a YPCXXC consensus sequence, which is implicated in the primary redox-reaction [58] and two additional cysteine pairs that are essential for correct function [59].
In addition, it should be noted that the SC barrier is a very dynamic structure and that apart from transcript levels also protein levels and posttranslational modifications affecting localization and interaction between the various components may be important for correct function.
The ROTEM® device was tested regularly for correct function using quality control serum (ROTROL®; Pentapharm GmbH).
Such mutations may be compensated for in nuclear encoded genes to allow for correct function (Rand et al. 2004; Osada and Akashi 2012).
If an in vivo implementation is intended, the cloned plasmids are verified for correct function in vitro before in vivo implementation.
Reasons for this diversity of results may be that at least some coding sequences carry structural features necessary for correct function, or that structure, no matter whether well-defined or ill-defined, was never selected for or against.
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One of the main technical limitations to consider is tolerance in the relative position of the various strips: for correct functioning, precision must be less than 0.1 mm.
This indicates that the linker region between MORN domains 6 and 7 is critical for correct functioning of MORN1.
Finally, we showed that the linker region between MORN domains 6 and 7 potentially serves as hinge critical for flexibility in the MORN1 complex and is a critical region for correct functioning of MORN1.
The ratio between the two constructs is crucial for correct functioning of the system as excess GFP-fusion protein that does not bind to the reporter mRNAs accumulates in the nucleus.
Nuclear compartmentalization is fundamental for correct functioning of the genome.
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