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This genotype would be the best material for sequencing the first genome of Saccharum, and even for that tetraploid genome, ultra long sequence reads from single molecules are needed for correct assembling of the homologous chromosomes and annotation of allelic variations with haplotypes varying from three to eight in homologous regions.
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Indeed, a physical interaction between co-translationally acting chaperones and ribosomes has been widely reported as the primary environment for the correct assembling of nascent polypeptides.
It is now well established that KCNQ1 channels assemble with KCNE β-subunits for correct physiological function; mutations that disrupt this complex formation result in congenital deafness and inherited cardiac arrhythmias [10], [37], [38].
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Look for correct crater size.
The 4 panels represent the Lorenz curves of individual user contribution to positive progress (i.e. the number of correct links assembled) for the different puzzles, and the corresponding Gini index.
Therefore, most of the research work focusing on PacBio reads uses the higher quality of SGS short reads, either for correcting the PacBio reads [ 25- 28] or for assembling in a de novo context [ 29, 30].
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This is a problem for assembling the correct set of complete mRNAs from a gene as information on the combinatorial relationships and possible coregulation of distant exons is lost, although these relationships can frequently be deduced by statistical inference.
For Vitrella, the reads were corrected and assembled followed by several base correction, scaffolding and gap filling steps as briefly described below.
However, Sanger sequencing via capillary gel electrophoresis is still commonly used to correct for errors in assembling the sequence data, for example in long repeats of DNA polymers.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com