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Attenuated total reflectance-infrared (ATR-IR) and Raman spectroscopy as powerful, non-destructive and easy-to-use techniques provide valuable molecular structure information and are convenient for verification of changes in the secondary structure.
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The obtained dependence of the nucleation rate on the concentration of monomeric protein is convenient for experimental verification and for use in rate equations accounting for nucleation-mediated fibril formation.
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CEO of Professional Science Editing for Scientists @ prosciediting.com