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The environmental status and socio-economic importance of the sites should be assessed both for continued cultivation but also for other ecosystem services such as restoration and hydrological functions (flood control).
With hybrid cultivation strategies, a dense inoculum is produced in photobioreactors with minimized risk for contamination and then introduced in raceway ponds for continued cultivation and biomass production (Tan et al. 2018).
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Part of the reason that opium production continues at record levels is that the Taliban profits greatly from continued cultivation.
We transferred EBs to gelatin-coated plates and continued cultivation for another 10 days (Fig. 5B).
BUT Ms. Story, who is herself black, sees black success on the operatic stage as something that requires continued cultivation.
So don't forget continuing cultivation — not just before the donation, but afterward as well.
When the optical density at 660 nm of the culture reached 0.5, 1 mM IPTG was added to the culture medium and cultivation continued at 30°C for four hours.
When the absorbance of 600 nm of the culture reached around 0.5, 1.0 mM isopropyl-β-D-thiogalactopyranoside (IPTG) was added to the culture medium and cultivation was continued for an additional 4 h.
After 6 h of cultivation, IPTG (1 mM) was added, and the cultivation was continued for an additional 2 h at 37°C.
The cultivation continued at 20 °C for additional 15 h.
The photosynthetic activity of H. cervicornis recovered and was above initial activity after a period of cultivation, but it decreased if cultivation continued too long.
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