Sentence examples for for constructing and selecting from inspiring English sources

Phage display is an alternative method for constructing and selecting antibodies with desired specificity towards an antigen.

Several excellent books provide detailed protocols for constructing antibody libraries and selecting antibodies from those libraries [5 13].

This cell line was then transfected with the TbCLK1-HA construct and selected for hygromycin and puromycin resistance.

To further validate these transient assays, rice callus was bombarded with promoter- pHLucGWgus constructs and selected for stable transformation.

We electroporated ku80Δ hpt mutant RH tachyzoites with the construct and selected for transformants in 50 μg/ml mycophenolic acid and 50 μg/ml xanthine.

H1299 cells were transfected with pcDNA3-TP53 full-length or pcDNA3.1TOPO-TP53-ORF pcDNA3.1TOPO-TP53-ORF pcDNA3.1TOPO-TP53-ORF500 μg/mL neomyconstructs

The resultant DLD1-7TGP stable cells were subsequently transfected with various myc-Daple constructs and selected for G418 resistance as described earlier in methods.

The GYTS follows a standardized methodology for constructing sampling frames, selecting schools and classes, preparing questionnaires, carrying out field procedures, and processing data [ 11, 12].

A simple rating methodology was applied, in which wetland images (N = 70) were rated for preference and selected connotative perceptual constructs on a 7-point modified Likert scale by participants (N = 241) recruited from community groups in Melbourne, Victoria's capital city.

Mammalian expression vectors (pcDNA3.1 construct; Invitrogen) for wild-type and selected PDB-mutant SQSTM1 proteins (His-FLAG tagged) were generated previously [22].

In the presence of hygromycin, the requirement for T7 RNA polymerase to drive expression of hyg removes the necessity for the continued use of G418 to maintain the pLEW13 construct and selects for trypanosomes with active T7 RNA polymerase.