Sentence examples for for constant expression from inspiring English sources

For constant expression we have put the T. reesei err1 gene under control of the constitutive pki promoter and transformed the construct into the fungal genome.

Four genes were tested for suitability as controls for constant expression across all the samples.

Finally, the CMV promoter might not be suited to function as a constitutive promoter for constant expression of transgenes.

To assure consistency in relating gene expression patterns to a housekeeping gene, GAPDH and two other housekeeping genes, β-actin and 18 S ribosomal RNA, were evaluated for constant expression across treatments.

Prior to selection of 16S rRNA as an internal standard, the expression of 16s rRNA of furfural-challenged and unchallenged C. beijerinckii 8052 cultures was analyzed and confirmed for constant expression under the reaction condition of the study.

5S rRNA (forward primer: 5′-gcccgatctcgtctgatct-3′, reverse primer: 5′-agcctacagcacccggtatt-3′) was used as the referent for miRNAs expression for its constant expression level across all samples and suitable size.

Without feedback of WUS to facX, facX could be exchanged for a constant expression of WUS.

qPCR conditions were 45 cycles as follows: 95°C for 4 s, 62°C for 10 s, and 72°C for 30 s. Normalization was done according to beta-2 microglobulin housekeeping gene (selected for its constant expression in the tissues tested), or according to a standard dilution curve on a c-fos mouse gene-containing plasmid when comparing targets amplified with different primers.

It seems that the Col2a1 promoter is a great choice for high constant expression in all chondrocytes, whereas the Col11a2 and Matn1 promoters govern a more distinct subset of chondrocytes but with weaker expression.

Only HSP 60 and beta-actin were successfully confirmed for their constant expression levels in both protein and transcript by western blot and qPCR respectively and therefore we excluded PDI as a potential candidate reference marker (see Additional file 5).

As previously reported by others [ 77], our results also showed that the 18S RNA gene was the most stable one for its constant expression levels and was finally chosen as the reference gene in our study.