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HIV status is assessed with the OraQuick Advance Rapid HIV-1/2 Antibody Test using oral fluid and OraSure Western Blot for confirmatory testing using oral mucosal fluids.
The biokitHSV-2 Rapid Test ("Biokit") is a readily accessible alternative to WB for confirmatory testing and can be performed easily on sera within a few minutes.
The expectation is that, as the technology matures, the need for confirmatory testing will diminish.
Assays using this method are simple to design and easy to perform, making them ideal for confirmatory testing.
Our method facilitates efficient use of the information available in pooled ELISpot data to avoid or reduce the need for confirmatory testing.
In the United States, a new HIV diagnostic algorithm has been proposed that uses an HIV-1/HIV-2 antibodifferentiationimmunoassayssay instead of Western blot or immunofluoresence for confirmatory testing.
The assay allows for the screening for the presence of both the nonstructural and envelope genes of both viruses simultaneously allowing for confirmatory testing to be done in a single assay.
Subjects who received a preliminary positive test result were immediately referred for phlebotomy for confirmatory testing with Western blot.
HI antibodies were observed in these three sera, albeit at titers one dilution below recommended cut-off values for confirmatory testing [9].
Delivery of test results, particularly for individuals who have blood drawn for confirmatory testing, will prove difficult among this transient population.
Isolate not available for confirmatory testing.
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