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Clearly, this is required for completing fusion and pore expansion.
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Since SNARE-dependent vesicle fusion is a Ca2+-activated process, it was tempting to speculate that TRPML may be the Ca2+-permeable channel responsible for providing the local Ca2+ elevation necessary to allow the "fusion-clamped" vesicles to complete fusion.
For a cluster size of R = 20 μm one obtains τlate ∼ 100 s, which is the time scale measured for complete fusion-site opening.
Samples characterized as fused ranged from incomplete fusions to complete fusions.
We next investigated whether the polyQ length might affect the duration of the fusion in the cell nucleus by determining the time needed for a complete fusion event.
The SHB of gp41 is an established target for N- and C-peptide fusion inhibitors [ 16– 20] as its complete formation is crucial for complete membrane fusion and pore formation [ 13– 16].
Finally, the epithelium disappears from the shelves, thus allowing for complete palatal fusion [ 1].
This core structure, termed the SHB (six helix bundle) is important for complete membrane fusion and pore formation [ 13– 16].
After adhesion of the bilateral palatal shelves in the midline, formation and subsequent disappearance of the medial epithelial seam (MES) are essential for complete palatal fusion.
As mentioned in the 'Introduction', lipid mixing (exchange between membranes) is necessary for subsequent content mixing (complete fusion), but it is not sufficient.
While this view is widely held by many people in the field, I think it would worth mentioning alternate views, in particular because work in Axel Brunger's lab has provided very strong evidence for formation of (partial) trans neuronal SNARE complexes between vesicles without fusion (vesicles can be docked through the SNAREs for 30 minutes without complete fusion!).
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