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See Additional file 2 for complete protocol.
For complete protocol details refer to the Additional file 1: Method S2.
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Samples (100 μL cell lysate) were then prepared for 2D-LC-MS/MS based proteome analysis using the filter aided sample preparation (FASP) kits (Expedion) with modifications to the protocol for simplification (for a complete protocol see [ 27]).
Based on these findings, we recommend the following elution conditions: The elution protocol assumes that a native immunoprecipitation in PBS-T (phosphate buffered saline + 1% Triton X-100) has been performed, using protein A sepharose beads to recover the immuno-complex (see File S1 for the complete protocol).
See Figure 5 figure supplement 1 for the complete protocol.
Validation of the array results was done using Applied Biosystems pre-designed qRT-PCR primer and probe sets (PDAR's, see ABI P/N 4333458_a.pdf for the complete protocol).
The difference in the explored costs for performing the complete protocol or not was €7.891 per department per year.
(For the complete protocols, evaluations, surveys and statistics for the project, see, Earth System Science Informal Education Network 2008).
We describe a complete protocol for atomically precise dangling bond (DB) logic gate construction on a hydrogenated Ge(0 0 1):H surface.
A complete protocol for this method is available (Supplementary Protocol S1).
Here we describe a complete protocol for a multiplex assay in single-tube reactions useful in pathogen diagnostics.
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