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Six cycles were required for complete homogenization.
Around 5-7 cycles homogenization were necessary for complete homogenization, which was verified by inspection on a microscope.
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Both the solutions were ultrasonicated for 10 min to ensure complete homogenization.
The sample was then mixed gently by vortex mixer and placed in a shaking water bath at 37°C for 15 min to ensure complete homogenization.
The water phase was collected and transferred to Qiashredder (Qiagen) columns and centrifuged (13,000 r.p.m. at room temperature) for two minutes to ensure complete homogenization.
Moreover, AgNO3 is poured (0.75 M and 1 M) into the mixture, keeping the oxygen-free environment, and then the solution was stirred for 30 min (in dark) to reach a complete homogenization.
The samples were then mixed gently by a vortex mixer and incubated for 20 minutes at room temperature to ensure complete homogenization.
Complete homogenization of the mixture was required to obtain good spectral profiles for leuconostocs.
Complete homogenization of type 2a inclusions in sample Gran-1b occurred at temperatures between 288 and 385°C, and there was a tight clustering of homogenization temperatures at 344 ± 15°C (Figure 7).
The worms were checked under a microscope to verify complete homogenization.
Afterwards, the samples were incubated at 45°C (below the temperature of the lamellar liquid-crystalline to hexagonal-HII transition of pure DEPE), with intermittent vortexing for 30 min to hydrate the samples and obtain multilamellar vesicles (MLV), followed by three freeze thaw cycles to ensure complete homogenization and maximization of peptide/lipid contacts with occasional vortexing at 45°C.
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