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For certain sampling sites, high contamination contents of the eels could be attributed to local sources.
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For certain samples, the concentration of TSS in the outlet was higher than the TSS concentration at the basin inlet, suggesting sediment re-suspension.
Comparisons using artificially degraded samples profiled with both the SNP multiplex and AMPFISTR® SGM plus™ (Applied Biosystems) demonstrated a greater likelihood of obtaining a profile using SNPs for certain sample types.
The environment containing both liquid and vapor can be advantageous for certain samples with respect to a system filled entirely with liquid on account of an effective decrease of the liquid thickness since the resolution is inversely proportional to the square root of the liquid thickness [12].
For certain samples, the available DNA was exhausted during library preps.
– For certain samples, some primer pairs were more successful than others.
Furthermore, the results from the power calculations for the MAQC dataset show that including the array effect can be slightly beneficial for certain sample sizes.
The lower sensitivity rates for certain samples and the possibility of a PCR-equivocal or PCR-inhibited result also mean that conventional culture and sensitivity testing should still be used at the same time.
Finally, qRT-PCR analysis was performed on the CSF2 and IL4 genes as these appeared to be differentially expressed on the arrays but their probe sets failed on flags for certain samples.
Molecular techniques still have issues that need to be dealt with such as their limits of detection and sensitivity for certain samples and certain situations, their correlation with adequate diagnostic gold standards, their clinical validation and the correct interpretation of results, and the risk of contamination.
These thresholds are assigned by adding/subtracting a value in M from a base line typically at M = 0. Determining suitable thresholds may be problematic in large sample sets with samples of varying quality and heterogeneity, often the case for tumor studies [ 10], and may result in setting too conservative thresholds for certain samples in order to avoid erroneous CNA calls.
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CEO of Professional Science Editing for Scientists @ prosciediting.com