Trace metal basis ultra-pure chemicals for buffer preparations were purchased from Sigma-Aldrich.
Thiobarbituric acid, CoA, N-ethylmaleimide, NAD+, NADH(H), NADPH(H), standards for oxidized and reduced glutathione, serotonin (5-HT), organic and inorganic components for buffer preparations, teraetoxypropane and butylated hydroxytoluene were purchased from Sigma Chemical Co (St. Louis, Mo, USA).
PFAA-free hay, PFAA hay, concentrate, distilled water used for buffer preparation and nylon bags were analyzed for contents of PFAAs.
Here, we report a simple, low-cost, and scalable method for buffer preparation using layer-by-layer assembly of boehmite nanoplates followed by thermal annealing.
Chemicals for buffer preparation, such as KH2PO4, KOH, Citric acid, and Trisodium citrate, were purchased from S.D. Fine Chemicals (Boisar), India, while Tris HCl was purchased from Loba Chemie, Mumbai.
In this study, we did not count the time spent for buffer preparation in the SDS and CTAB methods, but this time should be considered when extracting DNA from a few samples.
All other reagents for buffer preparation were purchased from Fisher Scientific.
All the salts used for buffer preparation were of analytical grade and purchased from standard suppliers (Merck, Fluka or Sigma-Aldrich).
Chemicals and solvents were all purchased from Sigma-Aldrich and ultra-pure water was used for buffer preparation (Milli-Q 18 MΩ, Millipore, France).
For the buffer preparation, the ultrapurified water with resistivity of 18 MΩ∙cm−1 obtained from Millipore Super-Q system (Millipore Co., Billerica, MA, USA) was used.
We used published aDNA protocols at McMaster, including the use of dedicated clean-room facilities for sample and buffer preparation, DNA extraction, PCR setup, and post-PCR work.
