To test the feasibility of employing a combined lung adenoma/skin papilloma assay for broader detection of chemical carcinogenesis than that realized with either bioassay done separately, four strains of mice, SENCAR, BALB/c, A/J, and ICR-Swiss, were administered carcinogens either by the oral or intraperitoneal (IP) routes.
The results demonstrate that the newly developed generic RT-PCR tests provide a relevant tool for broad detection and identification of tospoviruses in plant quarantine and diagnostic laboratories.
A universal PCR assay (LightCycler; Roche Diagnostics Corporation, Indianapolis, IN) that targeted the 16S rRNA gene was performed for the broad detection of microbes and for quantitative analysis, while an MRS-PCR assay that targeted the mecA gene was performed for specific detection of methicillin-resistant staphylococcus (MRS).
The ITS region has been shown a high degree of success for the broad detection of fungi (Buée et al. 2009) and was recently proposed to serve as the primary fungal barcode marker (Schoch et al. 2012).
Degenerate primers based on these two regions were designed and tested in a polymerase chain reaction-based assay for broad spectrum detection of members of this genus.
With the development of rapid molecular techniques, molecular assays, especially in a multiplex format, have been accepted as tests of choice for broad spectrum detection of respiratory viruses.
The application of the algorithm to VigiBase allowed identification of some cases of substandard medicines; however, some key prerequisites for broad prospective detection of substandard medicines are not currently fulfilled.
These primers hybridize to the 16S rRNA gene of essentially all pathogenic bacteria and are useful for broad spectrum detection of infection in situations where the infectious agent cannot be easily cultured, or in infections that can be caused by one of many different species that all present with similar, indistinguishable symptoms.
There are a number of attempts under way to develop technologies for broad-spectrum detection of infectious agents for clinical as well as biodefense applications [7], [8], [9], [10], [11], [12], [13], [14], [15].
