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The abundance of each subunit message relative to Slo3 message was determined for both testes (Fig. 4E) and sperm (Fig. 4F).
The reaction to surgery is presented as the mean value of reaction for both testes.
The median score for reaction to surgery was 5 for both testes, and the median score was 3 for quality of induction, anaesthesia and recovery, with a range of 1 3.
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Transverse side-by-side images of both testes should be obtained for comparison of echo texture, skin thickness, and color Doppler flow pattern.
For each animal, both testes, epididymides and the seminal vesicles, ventral prostate, and liver were excised and trimmed of fat.
Most boys (half or more of the boys in every age interval) had both testes "down," for a total of 31 of 44 boys (70%).
Of 58 relatives, one participant had a history of testicular torsion and orchidectomy; all other relatives had both testes available for analysis.
Thirty-five Finnish and 14 Danish boys who were scheduled for orchidopexy of one or both testes by a paediatric surgeon were included as cases.
Seven cases did not have orchidectomies (five with extragonadal germ cell tumours without clear gonadal primaries and two patients treated for metastatic disease without orchidectomy), both testes were examined by ultrasound in these seven cases.
Square regression (with mean lifespan as a dependent variable and Lim3A mRNA amount as independent variable) is a better approximation for our data (R2 = 0.062 for embryos; R2 = 0.011 for heads; R2 = 0.007 for testes) than linear regression (R2 = 0.0029 for embryos; R2 = 0.0006 for heads; R2 = 0.000 for testes).
Sperm was sedimented in a Sorvall centrifuge (RT 6000 D), 3000 rpm at 4°C for 10 min. For testes and ovary-specific samples, animals were selected and dissected as in [ 89].
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