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Results show that the MIPs prepared have a significant imprinting effect with a resulting imprinting factor of 3 for both templates.
It was shown that for both templates, the functional monomer acrylic acid (AA) provided the largest ΔE, while the functional monomer 4-vinylpyridine (4-VPy) provided the smallest one.
The PCR products were validated by agarose gel analysis which demonstrated bands at ~854 bp for both templates, accordingly to the size of the sequence.
For both templates, ligases are left to right: Tth DNA ligase (Thermo), Tsc DNA ligase (Prokaria), Thermostable DNA ligase (Bioline), T4 DNA ligase (NEB), T4 RNEB (NE.), E. coli DNA ligase (NEB).
Reliable quantification is still possible if the PCR extends into the linear phase, or even in the saturation phase, provided it is ascertained that the amplification efficiency is the same for both templates throughout the PCR, including the final cycles [ 12, 16].
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Replication bypass studies in vitro revealed that for both template sequences, Dpo4 had difficulty inserting dNTPs opposite the (6 S -HNE-1 S -HNE-1, N-dGuo adduct.
The replication bypass studies, in vitro, indicate that for both template-primers I and II, the Dpo4 polymerase inserts low levels of dATP opposite the (6 S -1R,11 S -1, N-HNE-dGuo adduct.
To compare our results with those of the LEAP method, we also tested the method on a benchmark used for the assessment of both template-based and ab-initio loop structure prediction methods in (Choi and Deane, 2010).
Data were obtained in both templates for Y206 mutations to ensure that the signal peptide did not affect receptor function.
The most striking Lsh target sequences at which DNA methylation is lost are repeat elements, which are both templates for the maintenance (Dnmt1) and de novo (Dnmt3a and 3b) methyltransferases in mice.
Thus, a key sequence is used for both the template and the query, and these two key sequences are synchronized.
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