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We generated 36-base reads from each allele starting at every possible position in each exon and repeated this process for both strands of DNA because RNA-seq is usually performed using double-stranded cDNA.
This proved difficult: nothing had been heard from Shackleton since Endurance had left South Georgia in December 1914, and it seemed likely that relief expeditions were necessary for both strands of the expedition.
The efficiency of cleavage was dependent on the IN concentration for both strands.
Cloned inserts were amplified and sequenced using the Macrogen™ (www.macrogen.com) sequencing facility, using M13 universal primers for both strands.
The sequencing procedure followed the one described above, but in this case all individuals were sequenced for both strands.
Nucleotide sequences were assembled for both strands and compared with existing entries in the MLST database (http://pubmlst.org/) for generation of allelic numbers and assignment sequence types (STs).
We found that frequency distributions of the length of gene clusters, continuously located on the same strand, have close values for both strands.
Relative to the previously established signal cutoff of log2 >0.73 based on background signals from probes for both strands of promoters of ∼4,600 verified Arabidopsis genes [82], it is apparent that Arabidopsis MIRNA hairpin expression was low for most probes.
All templates were sequenced in their entirety for both strands.
All amplified fragments were sequenced for both strands.
Ambiguous strands were counted as half reads for both strands.
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