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LD50 was done as per OECD guidelines for fixing the dose for biological evaluation.
Wizard 2480 gamma-counter and G4 microPET (Sofie) were used for biological evaluation.
Squamostolide and its related analogs were designed and synthesized for biological evaluation.
Novel C-aryl glucoside SGLT2 inhibitors containing pyridazine motif were designed and synthesized for biological evaluation.
Novel C-aryl glucoside SGLT2 inhibitors containing pyrimidine motif were designed and synthesized for biological evaluation.
To efficiently access asymmetric cis platinum (II) complexes for biological evaluation, a new solid-phase synthesis was designed.
Similar(28)
L929 cells were used for biological evaluations.
However, for datasets having calibration information available, using 3D landmark positions instead of projected 2D positions is desired for biological evaluations (e.g., [8]).
This material was used in subsequent conjugation reactions, but for biological evaluations of 4, a portion was purified by ionexchange chromatography on Q Sepharose Fast Flow resin with elution with TEAB (0 to 2.0 m) to give the triethylammonium salt of 4, which was accurately quantified by total phosphate assay.
The apparent low to moderate yields of the individual components are not considered as problematic since our primary interest is the rapid synthesis of a variety of peptide derivatives for initial biological evaluation, rather than high individual yields, commonly aimed for in methodological studies.
Compound 2h had a good inhibition selectivity for DPP-4 over DPP-8/9 and thus was selected for further biological evaluation, including oral glucose tolerance, plasma DPP-4 inhibitory activity, pharmacokinetic profile, acute toxicity and hERG inhibition.
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