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To my knowledge, this work uncovers a new mechanism for autolysin in preventing the detection of Gram-positive bacteria by host peptidoglycan sensors.
This would allows for the detection of fentanyl in drugs unbeknownst to consumers.
The detection of S. pneumoniae in the culture-negatives could be associated with the specificity of the cpsA gene primers used; hence, the inclusion of an alternate gene locus such as the autolysin gene (lytA) which has been shown to have high specificity could be used to verify the data presented here [ 46].
If anything, it seemed to increase the detection of a precancerous condition, D.C.I.S., for ductal carcinoma in situ.
The physicians at the Mount Kisco Medical Group strongly believe in the importance of mammography for the early detection of breast cancer.
The detection in shifts of gravitational waves.
Peter et al. (2009) developed a real-time PCR assay targeting the autolysin gene capable of detecting the equivalent of 125 CFU in 1 mL whole blood [ 49].
A similar behavior was observed for the lysogen in the absence of the bacterial autolysin.
The overexpression of autolysin genes involved in the restructuring of the cell wall further confirms these findings.
Consequently, the contribution of LytA, the main S. pneumoniae-specific autolysin, to the decrease of viable cells in late phase was examined, using the lytA-deficient mutant P103.
In the presence of vancomycin, five of the knockouts showed an OD620 reduced by 50% after 2 h, and a survival rate reduced by <2 orders of magnitude, in a similar fashion to the Δ lytA knockout (defective for autolysin).
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