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For appropriate references on abundance standards and related topics, see Snow (2000) and Draine (2003).
A previous study reported the validation and selection of appropriate reference genes for qPCR for protein coding genes [ 7], however, there is no report of stable miRNAs that can be used as reference genes in qPCR analysis of C. elegans miRNAs.
This strategy turned out to be successful in our hands for pre-selection of appropriate reference genes, even from a very limited set of transcriptomic data as starting material.
Thus, the choice of appropriate reference genes for normalization is a prerequisite for qRT-PCR assay.
Several recent studies have aimed to improve our analysis of the roles for microRNA in lung development, including a careful analysis of appropriate reference genes for real-time RT-PCR studies [45].
Finally, the selection of appropriate reference genes for normalization of quantitative real-time PCR has a major impact on data quality.
We further examine how the composition of reference gene sets affects the accuracy of the statistic, and suggest methods for selecting appropriate reference sets for any genome, including bacteriophages.
The main problem is that of identifying the variance for the appropriate reference population.
This measure was demonstrated in many studies to be valuable for selecting appropriate reference genes across several experimental conditions and treatments [ 45].
For each protein, appropriate reference complexes were selected from the PDB.
This paper addresses the current literature of multi-stakeholder system design, the ramifications of framing on MSTSE, considerations for establishing a more appropriate reference point, and example techniques and visual representations for doing so.
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