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The G22C MscL mutant was incorporated in fluorophore-filled vesicles and the channels were activated by adding the positively charged sulfhydryl reagent [2- trimethylammonium)ethyl] methanethiosulfonate bromide (MTSET), which reacts with Cys22 and thereby weakens the gate—allowing for channel activation without applied tension.
Choose an adjustable wrench and a crowbar for use as a lever to apply tension to the belt as you tighten it.
A special fixture was designed which allowed accurate positioning of the tibia for applying longitudinal tension to the graft.
This applies tension to the heavy, elastic intertransverse ligaments.
For our purposes, we use p open as it quantifies directly the probability of thermal fluctuations opening the hairpin, based on its sequence and the applied tension.
Weight of the PUD applies tension.
The principle here is slow, steady, gently applied tension.
The idler pulley should apply tension to the belt.
In combination with the specially adapted ER-rotors, the electrorheology system can be used for applying a high tension voltage.
CATIIQ device: CATIIQ is short for "Constant Applied Tension II Quick".
Pull it in various directions to add tension to the rope: Applying downwards tension allows the knot to keep tight.
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