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This issue is similar for any sequencing technology that relies on PCR amplification [ 55].
StatsDB features a generic database schema which enables the storage of data from any QC tool designed for any sequencing platform.
GemSIM, or General Error-Model based SIMulator, is a next-generation sequencing simulator capable of generating single or paired-end reads for any sequencing technology compatible with the generic formats SAM and FASTQ (including Illumina and Roche/454).
These simulations show that sampling around 100 mutants per animal is sufficient to achieve reliable mutation spectra with a power of 80%%, and therefore, pooling approximately 170 mutants per barcode (assuming a mutant recovery of ~60%%) should be more than enough for any sequencing application.
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For any sequence, and.
for any sequence in.
and for any sequence holds.
For any sequence in with, we have,.
for any sequences such that as and.
For any sequence with as, let.
For any sequence, we define (2.11).
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