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No Kenyan plasma samples were pooled for any assay.
This sparse time-lapse strategy should be useful for any assay where cell responses play out over a few days.
Plates were incubated for at least 30 min at 37°C and 10% CO2 for any assay that included antagonist, dye, or PAM modification to the aCSF.
For any assay there is typically more than a single way to express the results of the assay.
All hTERT-HMEC cells used were less than eight passages post immortalization, and primary PE cells were not cultured for longer than one week for any assay.
Readings below the limit of detection for any assay were arbitrarily assigned a value corresponding to 50% of the limit of detection to avoid division by zero in any subsequent calculation.
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Assay controls were incorporated onto the same plate, namely, no-template controls to test for the contamination of any assay reagents.
These should be tested in quality-assured laboratories using liquid culture which has the highest sensitivity for Mycobacterium tuberculosis of any assay.
First, we completely agree that for a thorough judgment of any assay samples from clinically well-defined patients are necessary.
We are not aware of any validated correction factor for hemodilution for endotoxin levels with any assay as endotoxin exists in many forms and compartments in vivo and the impact of hemodilution on each of these is unknown.
Any assay for which the accepted control criteria were not met was discarded and the assay repeated.
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