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These innovations were also highly beneficial for antibody expression.
Ig sequences were transfected into HEK293 cells (Expi293F™ Cells, Gibco, Cat. No. A14635) for antibody expression as described below.
The vectors for antibody expression and display in TG1 cells were derived from pBluescript SK.
The Ig-cassette contains all the essential elements for antibody expression and V-gene-specific long homology overlaps within a single DNA molecule.
However, sialic acid is incorporated into antibodies at low levels: it is present in 25% of polyclonal IgG in human serum and in less than 5% of antibody glycans in cell lines commonly used for antibody expression.
Cells tagged with antibody expression constructs were cloned into 96 well plates after selection of hygromycin resistance and then tested for antibody expression by ELISA and for GFP by FACS.
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In this study, we explored strategies for optimization of antibody expression in general laboratory strains of E. coli using simple methods for reducing translation rates.
Purified scFv was used as a standard for the quantification of antibody expression in pea seeds by ELISA using Eimeria oocyst extract as an antigen.
Here, we compared different forms of the same HIV-1 strain R2 Env for antigenic and biophysical characteristics, and in rabbits characterized the extent of B cell induction for specific antibody expression and secretion and neutralizing responses.
For transient antibody expression, HEK293T cells were transfected according to the Ca3(precipitationtation method [ 46].
This strategy was adapted to account for variable antibody expression through the use of hybrid magnetic/plasmonic nanocarriers modified with several different antibodies.
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