Sentence examples for for antibody background from inspiring English sources

Discs containing null clones were used for antibody background subtraction.

As controls for secondary antibody background and autofluorescence, slides were subjected to this protocol omitting the primary antibody.

Controls for specificity included staining neurons with primary antibodies without fluorescence-conjugated secondary antibodies (background controls) and staining neurons with only secondary antibodies.

For each antibody, the background was subtracted (determined by a negative control consisting of a secondary antibody alone).

For each antibody subtract background intensity from values and then divide by the γ-tubulin loading control.

A. For each antibody subtract background intensity from values and then divide by the GAPDH loading control.

Imaging analysis involved a novel quantification algorithm that corrects for non-specific antibody binding, background signals and differences in overall staining intensities between fibres using appropriate normalization routines.

Controls included: a positive control, mouse brain sections incubated with each of the above antibodies; negative controls omitting the primary antibody; for background staining the cells and tumors were incubated with normal rabbit serum.

For antibody details, see below.

Background staining for antibodies was determined in negative cell lines and with matched flurochrome-conjugated isotype controls.

Background staining for antibodies was performed in negative cell lines and with matched fluorochrome-conjugated isotype controls.