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From the slope of the Scatchard plot, given in Fig. 3C, the association constant, Ka was estimated as 3.09×104 M−1, whereas the X-intercept of the plot was obtained as 1.2, indicating that each molecule of PDC-109 has one binding site for ANS, which is comparable with ANS binding to α-crystallin [47], [48].
The samples for ANS fluorescence measurements were prepared by adding 1 75 ANS probes to the protein solutions.
For ANS fluorescence measurements as a function of pH values, the protein concentrations were 30 μM and that of ANS was 20 μM.
The affinity of BS is higher (Kd=3.2 μM) than that for ANS (Kd=195 μM).
Moreover, we present a new common and flexible code distribution scheme for ANs, named as code distribution scheme (CDS).
A method to screen for ANS dysfunction in symptomatic pediatric patients who have been referred to a neurologist in order to reduce cases of misdiagnosis and improve treatment outcomes.
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