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In the context of HTS assays, the target label set of a particular assay is just considered as an extra-added feature for another assay that follows the chain order.
However, this method requires cell solubilization, and the sample cells are killed and, thus, cannot be used for another assay.
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This assay uses the same antibodies as the assay previously used in dogs (Diagnostic Systems Laboratories), but with standards for the calibration curve from another assay (Immunotech) [ 22], and it measures AMH in human, monkey, bovine, dog and other mammalian species [ 25, 26].
If both sensitivity and specificity increases for RA it might provide another assay for diagnosis of the disease.
The primers used for the qPCR studies, including one assay for the total FBXO7 transcripts (isoform 1 and isoform 2), one assay specific for the isoform 1, and another assay specific for the isoform 2, are given in Supplementary Table S2.
For example, in a comparative study with FAIRE-seq, another assay for mapping open chromatin sites, some open chromatin sites are unique to either DNase-seq or FAIRE-seq.
This experiment also led to another assay for silica particle uptake.
Thus a deletion detected with a single probe set needs to be confirmed with another assay, for example with quantitative PCR.
In another assay for surface presentation of the KCNE proteins, intact cells were subjected to surface-labeling with biotin followed by streptavidin-precipitation prior to cell lysis and SDS-PAGE analysis.
We also examined how disruption of the Pat1C-Lsm2-3 interaction affected 3′ to 5′ degradation of the mRNAs by the exosome, which increases when decapping is inhibited and thereby provides another assay for the in vivo function of the Pat1-Lsm1-7 complex.
Another assay specific for fragments of the collagen type I C-telopeptide, ICTP, results primary from nonosteoclastic matrix-metalloproteinase-mediated degradation of type I collagen [ 34].
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