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The stirring process was continued for another 3 hours, and then the mixture was transferred to a Teflon-lined stainless steel autoclave and heated at 190 °C for 24 hours.
Expressions of MBP or MBP-fusion proteins were induced by adding 0.5 mM IPTG and incubating at 24°C for another 3 hours.
The other half of the material was incubated for another 3 hours with a solution containing coelenterazine, and tested in the same way (Figure 6D).
In this case, previously to the incubation with TTR, A-Beta peptide was incubated alone for aggregation purposes, and then further incubated in the presence or absence of TTR for another 3 hours.
After allowing 3 hours for transfection, the cells were washed and treated with bruceantin for another 3 hours and then assayed for pMHC I expression with the MYL8 or LYL8/Kb-specific BCZ103 T cell hybridoma (Fig. 7B).
After incubation for another 3 hours at 37°C, the retroviral supernatant was replaced.
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For another 16 hours.
But only for another 24 hours.
Stir one more time, then freeze for another 3-4 hours.
"We'll watch you for another 24 hours.
Sleep deprivation was paused for two days, and then resumed for another 21 hours.
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