Exact(8)
Leave to rest for 10 minutes and knead for another 10 seconds.
They're allowed to cook unmolested for another 10 seconds, before I then dive in again with the spoon, and so on, until they're just set, with big, soft curds.
This was followed by amplification of the target DNA through 45 cycles of denaturation at 95°C for 10 seconds, annealing at 58°C for 10 seconds and elongation at 72°C for another 10 seconds.
For fluorescence signal acquisition, time and temperature profile were set as follow: holding step at 95°C for 15 minutes for enzyme activation, 40 cycles starting in denaturation step at 95°C for 10 seconds, annealing at 58°C for 10 seconds and lastly extension step at 72°C for another 10 seconds.
Hold this for another 10 seconds.
Mix the jelly and then melt for another 10 seconds.
Similar(52)
It is suggested that you put mix in the microwave for 15 seconds, stir another 10 seconds, and do it again until it's a thin paste.
Return chocolate to microwave on high for another 5 seconds.
Add the scallops and cook for another 20 seconds.
Add the cilantro and stir-fry for another 30 seconds.
Pause for another 20 seconds, then repeat the gentle stirring-and-folding process.
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