A range of reach-scale indices are derived from the RHS database for analysis, supported by landscape variables which provide detail on the catchment context.
But for this commentary, we emphasize a case approach, using four respondents for analysis, supported by some commentary from others in the discussion section.
This raises the issue of potential bias and when not controlled for in analysis supporting policy decisions, can lead to poor allocation of public resources.
RWK was responsible for analysis support, manuscript development, and final manuscript review.
We designed 147 PCR product length polymorphism (PLP) primers, as described by Kimura and Naruse (2010), for QTL analysis (supporting information, Table S1).
After genotype calling and further quality filtering (SNPs with double reduction, segregation distortion, and missing values), there were 1972 SNPs available for linkage analysis (Supporting Information, Table S1).
The data set used for phylogenetic analysis supporting results of this article is available in Dryad, DOI number: doi: 10.5061/dryad.j4489 [ 76].
Median values were used for all further analysis (Supporting Information, File S5 and File S6).
Under this CPM cutoff, 13,593 genes were eligible for allelic expression analysis (Supporting Information, Table S1).
The WBGR approach turns a nuisance in straightforward multilevel regression analysis into an advantage for in-depth analysis supporting or refuting causal claims in cross sectional analysis.
For ML analysis, supports for nodes among branches were evaluated using nonparametric bootstrapping [ 17] with 1000 bootstrap replications.
