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(C) In vitro binding assays for analysis of the binding of recombinant KDM5B variants to the unmodified histone H3K4 N-terminal tail.
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We applied yeast two-hybrid and in vitro binding assays as a proof-of-concept trial for analysis of the newly identified proteins for binding to Hsp90.
For analysis of CoA-binding, the final cell CoA concentration and protein concentrations were recalculated based on estimates of the CoA concentration obtained by binding of ADC-T57V.
A separate set of samples for analysis of GR binding capacity was surface stained as described above but not permeabilized.
We also performed genome-wide analysis of the binding sites for the Hox cofactor Homothorax (Hth), revealing a striking similarity with the Ubx binding profile.
A role for tissue-specific Ubx binding is supported by a global analysis of the binding sites.
Structural analysis of the binding pocket identifies important intermolecular contacts that mediate binding.
For analysis of cardiac ACE binding, results were log-transformed to stabilize variance.
Peptide phage display is a powerful tool for the analysis of binding specificities of peptide binding domains [ 47].
Protein arrays are thus useful tools for the comparative analysis of binding specificities of peptide binding modules.
The binding analysis showed the dissociation rate for the binding of BtA to its receptors (7.495 × 10−3 S−1) was twice slower than that of Bt toxin (1.695 × 10−2 S−10−2
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