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For analysis, activated charcoal particles were taken from the samplers and then immersed in 2 mL carbon disulfide (Wako Pure Chemicals, Osaka, Japan) in a glass tube with a screw cap.
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In the analysis, activated Tregs were defined as CD4+Foxp3+CD25hi.
Mutational analysis for activating KIT and PDGFRA mutations is strongly recommended in KIT-negative tumors and in all advanced GISTs.
16, 23, 39 Mutational analysis for activating mutations is strongly recommended in all advanced GISTs because of their predictive value.
Analysis of activated Caspase 3 provided consistent results (Fig. S1).
The final dataset used for analysis included 408 activating, 1932 neutral, and 9736 deleterious mutations.
Within this subgroup, we chose AKT2 for analysis because AKT2 phosphorylates activating transcription factor 2 (ATF2) [ 14, 15].
Using the HPT method, we demonstrate that inducers in the agarose hydrogel substrate regulate gene expression of the patterned cells for qualitative analysis by activating the promoters of fluorescence protein genes.
The software response can be displayed on the monitor screen or transmitted over a serial port for further analysis or for activating electro-mechanical actuators.
(B ) Structure-activity analysis of activating compounds.
Additionally to the in silico pathway analysis, to look for differentially activated pathways following 17AAG treatment we have applied transcription factor-responsive luciferase reporter assays to resistant (T47 D, MDA-MB-231) and two sensitive cell lines (Hs578T, MCF-7).
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