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Samples were washed twice in ice-cold PBS, then resuspended in PBS/1% FBS and 5 μl of FITC-conjugated secondary antibody (anti-mouse IgG-FITC, Sigma-Aldrich, Singapore) was added, and incubated in dark for an 30 min at RT.
Similar(58)
For an 11-inch TV.
"Not for an 80-year-old.
Not bad for an 11-year-old.
Good entertainment for an 11 year old".
For an 84-0 loss against then-No.
Prius for a 100-m.p.g.
He "settled" for a 63.
BMW Z3 roadster for a 25-m.p.g.
Then again for a 14-yard loss.
Hefeweizen, $6 for a 22-ounce glass.
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