Exact(2)
We studied the total SFS (considering pooled samples from all populations); in the main text, we present results for equal sample sizes in each populations, and we present results for alternative sampling schemes.
Simulations were used to estimate the coefficients of variation (CVs) of population density estimates for alternative sampling designs, a model was developed to determine the optimal survey design which minimised survey time for a given survey precision and a statistical power analysis was used to determine whether these surveys were sufficiently sensitive to form part of a monitoring programme.
Similar(58)
Illumina protocols recommend 10 cycles of PCR when starting with one microgram of input DNA, and 18 cycles when starting with 5 nanograms, but it is difficult to know a priori how to optimize cycle number for alternative sample amounts.
Simple random sample will serve as the baseline for comparing alternative sampling designs.
Moreover, urine can be used as alternative sample for DNA extraction that can be employed for the multiplex PCR method described in this study.
Figure 1 presents the fraction of individuals self-identifying as white by alternative samples for 1994-2012.
Results for the alternative samples are available upon request.
We hence run our Mincer regression for an alternative sample that excludes individuals below the age of 30 (see Table 21 in the Appendix).
A surface flowing mode sample holder was designed as an alternative sampling strategy for direct analysis in real time mass spectrometry (DART-MS).
Even this strategy was not sufficient to attain the targeted sample size within the expected calendar, so eventually spatial sampling and random routes were also used to complete the sample (see Alternative sampling methods for immigrants in Spain and Italy ).
Circulating free fetal DNA can be used as an alternative sample for prenatal paternity tests because of its similarity with maternal DNA.
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