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We are grateful to Northwestern University's Genomic Core for all sequencing completed for this project.
Supplies from requester: For all sequencing and fragment analysis services, they provide some combination of sample and primer.
We thank L. Gaffney for help with figure preparation, J. Bochicchio for project management, the Broad Sequencing Platform for all sequencing work, A. Papanicolaou and M. Ott for Inchworm software testing and code enhancements, and F. Ribeiro for helpful discussions regarding error pruning.
Therefore, the removal of sequence contaminants is a necessary and required step for all sequencing projects.
For all, sequencing and assembly of the shotgun data was performed using a standard whole genome sequencing assembly with the 454/Roche Newbler assembler V 1.1.02.15.
Clustering and sequencing of the material was carried out as per manufacturer's instructions – v2 Single Read Cluster Kits and v3 SBS kits (Illumina Inc, San Diego, Ca, USA) were utilized for all sequencing.
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MiSeq from Illumina was used for all sequence analyses.
One curve generally isnt good enough for all sequences.
The start codon and/or stop codon could not be found for all sequences (Fig. 1).
The laser energy and the auto-focus are controlled for all sequences used for calibration.
The system is quite capable to generate manufacturing drawing of die sets for all sequence of operations.
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