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Laser power was set at 6% with a constant gain setting of 780, for all quantification acquisitions.
Standard deviation was determined for all quantification measures.
Briefly, the muscle 4 NMJ of abdominal segment 3 was used for all quantification.
The dsDNA dye SYBR Green I (Molecular Probes, Eugene, OR, USA) was used for all quantification (1/50,000 final).
The thermal cycling conditions were identical for all quantification experiments, i.e. after the first cDNA synthesis at 50°C for 30 min, DNA polymerase was activated at 95°C for 10 min, followed by 40 cycles of denaturation at 95°C for 20 s, annealing at 55°C for 30 s and extension at 72°C for 40 s.
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For all the quantifications an average number of 3 sections was counted per rat spanning about 500 µm along the antero-posterior axis.
Summary of all quantification metrics for all patients.
For all quantifications, three sections of all animals and five animals of each group were used.
For all quantifications, data (mean ± SEM) were from 3 independent experiments.
The statistical significance for all quantifications was assessed by two-tailed unpaired Student's t tests and is indicated by asterisks in the histograms and figure legends.
ImageJ was used for all quantifications.
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