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Through the ClustalX software (http://www-igbmc.u-strasbg.fr/BioInfo/), all amino acid sequences of Rab proteins obtained were used for alignment analysis (Thompson et al. 1994).
The programs JALVIEW2.4 [30] and TREEVIEW [31] were used for alignment analysis and tree visualization, respectively.
For alignment analysis, U2OS cells plated on 15 mm coverslips were treated and fixed with 4% Shandar Zinc Formal-Fixx™ (Thermo electron corporation) for 20 minutes.
The sequence databases used for alignment analysis were obtained from NCBI.
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For TBLASTN alignment analysis, amino acid sequences from Pseudomonas aeruginosa PAO1 for each of the 11 genes were used as a query in a TBLASTN (BLAST+ v2.2.23) search of the Can.
This algorithm allows the systematic fragmentation of metabolic pathways for the alignment analysis, and it has been used to identify the shortest pathway between compounds in metabolic networks [ 21].
The AACT sequences of other 11 species of highly homology from GenBank were taken for multiple sequence alignment analysis (Fig. 2).
Thirteen sequences of the SrMV CP gene and four sequences of the SrMV whole genome in NCBI were selected for multiple sequence alignment analysis.
The sequence alignment analysis for all SNPs and the designed assays in human genome was performed using BLAST.
They were used to conduct alignment analysis for preliminary species identification in the NCBI genomic database (http://blast.ncbi.nlm.nih.gov/) and CBS database (http://www.cbs.knaw.nl/).
Ten SSR loci were randomly selected for DNA sequencing, and sequence alignment analysis revealed nucleotide variation of the SSR motifs and repeat lengths among Orchidaceae taxa.
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