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With the redundancy of the overlapping probes in the lettuce array, the need for additional replication was reduced because they provide technical replicates within a chip.
Also, an independent cohort of 549 CRC cases and 599 controls outside EPICOLON was available for additional replication.
We also took four bivariate signals forward for additional replication, which were just below the genome-wide significance threshold, but none were robustly associated in both traits.
Weak but consistent evidence for a protective effect for haplogroup E1b1b1c was seen in all studies with a nominally significant meta-analysis, thus, calling for additional replication efforts for this haplogroup in populations of Ashkenazi Jewish and European ancestry.
The need for additional replication may also have been reduced by using elevated amounts of genomic DNA and the use of end-labeling rather than BioPrime may have increased the reliability of calls.
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An additional replication dataset collected in France contained 113 participants.
For H. volcanii, in addition to the five replication origins that were previously genetically mapped [ 9], five additional replication origins were also predicted in this study.
These weak positives require additional replication study.
A meta-analysis supported an association between the HTR1B gene and ADHD (pooled odds ratio ~1.44; [ 53]), and although there are subsequent additional replications for HTR1B [ 70, 137], there are again also negative findings [ 21, 77].
In the geometric replication mode (GR), newly made progeny genomes are used to create further templates for additional rounds of replication within a single cellular infection cycle.
Saturation was reached after 50 bootstrap replications, with additional replications not yielding significantly different results.
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