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Ethylenediamine reacts with Ge(100 -2 100 -2 produce a surface-bound amine group which is available for additional reaction.
Alternatively, photoactive protein Z constructs have been made by post-translationally modifying a recombinant protein, but the need for additional reaction and purification steps complicates production and limits the final yield.
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A contextual menu is provided, which allows the user to (i) remove a compound from the graph, along with all associated links; (ii) search the reactions database again for additional reactions involving the compound and (iii) search for the compound within the Enzyme List.
For additional reactions, see our Golden Globes liveblog coverage.
The introduction of surface amine functionality to the cellulose nanocrystal allowed for an additional reaction with a biobased epoxy resin derived from diphenolic acid.
A mixed integer linear program was used to determine the minimal set of additional reactions required for the production of biomass.
The PCR procedure employed was as follows: primary denaturation for 5 min at 94°C; 30 cycles of denaturation at 94°C for 30 s; annealing at 55°C for 30 s, and extension at 72°C for 100 s; and an additional reaction for 10 min at 72°C.
We propose a mechanism for this unforeseen additional reaction, and demonstrate its evolutionary conservation in bacterial orthologs of mammalian NAMPT and NAPT.
Double-stranded cDNA was synthesized using an E.coli DNA polymerase I (NEB) with RNase H (Invitrogen) at 16°C for 1 hour followed by an additional reaction for 1 hour at 22°C with T4 DNA ligase (NEB) treatment.
An additional reaction for β-actin was included for each cDNA.
This reaction expands the range of the products which can be formed, solves the problem of esters being unstable to the basic methanol conditions, and introduces the potential for many additional reactions of the more oxidized carbonyl group in the product.
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