There is still a need for the development of additional expression hosts from less studied phyla and of robust shuttle and induction systems for these hosts.
For the additional expression verification of five germline-specific genes identified by our bioinformatic screen, we performed RT-qPCR (see Supplementary Information) using the GoTaq PCR Mastermix (Promega, Madison, WI) and the ABI 7300 qPCR machine.
To select transcripts for further analysis, an additional expression cut-off of twofold was applied.
To check if this interaction was influenced by TRAF3 or TRAF5, we examined the effect of cotransfecting an additional expression plasmid for TRAF3 or TRAF5.
For the same reason, as additional expression of ispF increased the level of produced carotenoids significantly, it is suggested that ispF has a larger contribution to the flux control in the MEP pathway than ispD.
Additional expression analysis for comparison of other genes in the PNMA gene family, PNMA1, PNMA2, PNMA3, and MOAP1 (PNMA4), showed that they were widely expressed across areas and layers but without the differentiated pattern of PNMA5.
These results can be used as input to DESeq (Anders and Huber, 2010), for example, for additional differential expression analysis.
We searched for additional gonad expression data for the specific group of retrogenes and their parental counterparts analyzed by Metta and Schlötterer [ 21].
Dry matter intake and average daily gain described elsewhere [ 8] were used to decompose RFI via the regrouping of the animals based on these traits for additional gene expression analyses.
