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As co-Editors-in-chief with long experience in this field, we exercise our judgment concerning the necessity for additional experiments proposed by referees that would otherwise delay publication of an exciting result and/or represent an unreasonable amount of work.
It was then possible to choose levels of the factors for additional experiments to reach response targets.
Mass-transfer parameters were estimated using existing correlations in the literature without the need for additional experiments.
The researchers also noted that this new class of controllers could be developed from simulations based on experimental data, with no need for additional experiments for calibration.
These studies provide the groundwork for additional experiments into the role that this protein may be playing in viral pathogenicity, persistence, and cell tropism.
For additional experiments, we defined vehicle types by sedan, SUV, and truck.
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The detailed methods for the set of additional experiments are reported in the Additional file 1.
For brevity, we only report the results and a tabular summary of methods for the additional experiments (i.e. those which, although not originally designed for testing costs, could nevertheless be used for that purpose).
Supplementary Fig. 2 shows the strength profiles for the rheology used in Figs 2 and 3 (Supplementary Fig. 2a), as well as for the additional experiments (Supplementary Fig. 2b d).
After decross-linking, proteinase K, and RNase treatment, the immunoprecipitated DNA was purified by phenol/chloroform extraction for the additional experiments including Illumina single-end sequencing22,45.
Thus, 10 µM LY294002 was utilised for all additional experiments.
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