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Note that a small in silico fragment is considered as missing if this condition allows for a valid alignment that improves the local χ on nearby matches by half (up to three consecutive fragments).
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These two fixed genes guarantee generating a valid chromosome, and therefore a valid alignment.
This approach has a constraint, as it follows a greedy strategy: it finds a valid alignment if one exists, but this might not be the best alignment.
There are two restrictions on each RB to make a valid alignment, they are as follows: 1.
We used the −k = 1 option in BowTie2, which reports a single valid alignment for each read in the library.
Bowtie2 by default performs a non-exhaustive search for distinct valid alignments, and then reports the single best result of the identified alignments.
We then precompute P(r n | I n ) only for the valid alignments.
The reads were mapped to the E. grandis v.1.1 reference genome [ 47] using Bowtie2 [ 68] in Galaxy [ 69, 70], using parameters: "sensitive" pre-set option, 50 1,000 bp insert size for a valid PE pair, end-to-end alignment.
Since the first release of the program DIALIGN in 1996, a 'direct' greedy approach has been used where local pairwise alignments (fragments) are checked for consistency one-by-one to see if they can be included into a valid multiple alignment.
Next the reads were aligned against the HOX cluster allowing for no mismatches, all valid alignments for each read were reported.
An alignment was considered valid if there were two or fewer mismatches relative to the reference sequence (-v 2) and a read was required to have only one valid alignment (-m 1).
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